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Immunohistochemical methods for the visualization of Mycobacterium paratuberculosis in bovine tissues
A R Massone1, A A Martín, G S Ibargoyen
1Institute of Pathology, Faculty of Veterinary Sciences, La Plata, Argentina.
Abstract:
The peroxidase-antiperoxidase (PAP), streptavidin-biotin (SB), and avidin-biotin-complex (ABC) techniques have been evaluated for the visualization of Mycobacterium paratuberculosis (Mp) in formalin-fixed, paraffin-embedded bovine tissues. The used immunoperoxidase techniques were comparatively better than the Ziehl-Neelsen stain, specially for the demonstration of small number of mycobacteria in tissue sections.