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Updated: May 10, 2026

One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
Published on: June 18, 2016
Bioactivity and immunological evaluation of LPS from different serotypes of Helicobacter pylori
Davoud Esmaeilli1, Ashraf Mohabati Mobarez, Ali Hatef Salmanian
1Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran ; Department of Bacteriology, Faculty of Medical Sciences, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Background And Objectives:
Helicobacter pylori is the causative agent of peptic ulcer disease and a co-factor in development of gastric malignancies. LPS are among toxic substances produced by H. pylori exhibiting low endotoxic activity compared to typical bacterial LPS. The aim of this study was to investigate bioactivity of LPS produced by different serotypes of Helicobacter pylori compared to Escherichia coli and Brucella abortus LPS.
Materials And Methods:
Bacterial LPS was extracted by the hot phenol-water method. Biological activities of LPS were determined via the limulus lysate assay, pyrogenic assay, and blood pressure and PBMC induction test in rabbits.
Results:
Biological activity of O2 serotype LPS of H. pylori was less than the biological activity of other H. pylori serotypes.
Conclusion:
Our data supported the hypothesis that the unique bacterial LPS of the O2 serotype must be included in the formulation of a multivalent H. pylori vaccine.
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