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Related Experiment Video

Updated: May 10, 2026

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
09:28

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Published on: February 2, 2013

Real time assays for quantifying cytotoxicity with single cell resolution.

Sonny C Hsiao1, Hong Liu, Taylor A Holstlaw

  • 1Adheren, Inc., Berkeley, California, United States of America. sonny@adheren.com

Plos One
|July 5, 2013
PubMed
Summary

A novel live cell assay platform enables real-time cytotoxicity measurement in whole blood. This method accurately quantifies complement-dependent and antibody-dependent cellular cytotoxicity without hazardous chemicals, streamlining antibody drug discovery.

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Last Updated: May 10, 2026

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
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Published on: February 2, 2013

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08:27

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Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level
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Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level

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Area of Science:

  • Biotechnology
  • Immunology
  • Drug Discovery

Background:

  • Cytotoxicity assays are crucial for evaluating antibody-based therapeutics.
  • Existing methods often require hazardous radiochemicals and lack real-time, single-cell analysis.
  • Assessing efficacy in whole blood provides a more biologically relevant context.

Purpose of the Study:

  • To develop a novel, live cell-based assay platform for determining cytotoxicity.
  • To enable real-time, single-cell analysis of complement-dependent cytotoxicity (CDC) and antibody-dependent cellular cytotoxicity (ADCC).
  • To facilitate cytotoxicity assessment using human whole blood, bypassing the need for radiochemicals.

Main Methods:

  • Development of a live cell array platform using fluorescent Cell Tracker dyes and DNA-based immobilization.
  • Quantification of cell death via propidium iodide staining and automated microscopic imaging.
  • Utilizing whole blood or peripheral blood mononuclear cells (PBMCs) with antibody and serum/complement components.

Main Results:

  • The platform successfully determines CDC, ADCC, and overall cytotoxicity in human whole blood.
  • Achieved excellent batch-to-batch reproducibility for reliable results.
  • Enabled real-time, single-cell cytotoxicity assessment, identifying resistant cell populations.

Conclusions:

  • The developed assay platform offers a convenient, safe, and accurate method for cytotoxicity evaluation.
  • This approach significantly streamlines the drug discovery process for antibody-based therapeutics.
  • The use of whole blood enhances the biological relevance of the cytotoxicity assessment.