Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 10, 2026

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

Retinal Thickness Normative Data in Wild-Type Mice Using Customized Miniature SD-OCT.

Lee R Ferguson¹, James M Dominguez, Sankarathi Balaiya

¹Department of Ophthalmology, University of Florida College of Medicine, Jacksonville, Florida, United States of America.

|July 5, 2013

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Treponemal Endovasculitis leading to branch artery occlusion: a rare manifestation of ocular syphilis.

Oxford medical case reports·2026

Same author

Inexpensive 3D-Printed Adapter for Wide-Angle Viewing Contact Lens System in Vitreoretinal Surgery.

Journal of vitreoretinal diseases·2026

Same author

Incidence of fluorescein angiography-proven cystoid macular edema after 4-point Gore-Tex scleral fixation of the Akreos AO60 intraocular lens.

Journal of cataract and refractive surgery·2026

Same author

A Review of Recent Developments in Artificial Intelligence and Big Data Technologies for Ophthalmology Referrals and Clinical Practice.

Medical science monitor : international medical journal of experimental and clinical research·2026

Same author

Comparison of Retinal Thickness Measurements Using Optos Monaco and Heidelberg Spectralis OCT Across ETDRS Sectors in Normal Eyes.

Tomography (Ann Arbor, Mich.)·2025

Same author

Efficacy of inverted internal limiting membrane flap for treating optic disc pit maculopathy.

Journal of surgical case reports·2025

This study provides normative retinal thickness data for C57BL/6 mice using SD-OCT. These findings establish a standard for retinal morphology in this common animal model.

Area of Science:

Ophthalmology
Animal Models
Retinal Imaging

Background:

The C57BL/6 mouse is a widely used model in vision research.
Establishing normative retinal thickness data is crucial for interpreting experimental results.

Purpose of the Study:

To establish normative data for retinal thickness in wild-type C57BL/6 mice.
To utilize a miniature Spectral Domain Optical Coherence Tomography (SD-OCT) system for precise measurements.

Main Methods:

Adult C57BL/6 mice (3-5 months) underwent anesthesia for SD-OCT imaging.
Images were standardized by centering the optic nerve head (ONH).
Global, quadrant, and sublayer retinal thicknesses (inner retinal layer [IRL] and outer retinal layer [ORL]) were measured.

More Related Videos

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

Published on: September 22, 2017

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy

Published on: January 12, 2022

Related Experiment Videos

Last Updated: May 10, 2026

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

Published on: September 22, 2017

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy

Published on: January 12, 2022

Main Results:

The overall mean global total retinal thickness (TRT) was 204.41±5.19 µm.
Mean TRT values varied slightly across quadrants (inferior, nasal, temporal, superior).
Mean ORL thickness was 126.37±10.01 µm and IRL thickness was 107.03±10.98 µm, with detailed sublayer measurements provided.

Conclusions:

Normative values for total and sublayer retinal thickness in C57BL/6 mice were successfully established.
This data provides a standard for retinal morphology in this model.
The findings aid in evaluating therapeutic interventions and understanding retinal disease pathophysiology.