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Bioinformatic analysis for structure and function of TCTP from Spirometra mansoni
Ya-Jun Lu1, Gang Lu, Da-Zhong Shi
1Department of Pathogen Biology, Hainan Medical University, Haikou, PR China. luyajuncc@163.com
Asian Pacific Journal of Tropical Medicine
|July 6, 2013
Summary
Bioinformatics analysis of Spirometra mansoni translationally controlled tumor protein (TCTP) reveals its structure and function. This parasitic worm protein may serve as a vaccine candidate and drug target.
Area of Science:
- Parasitology
- Bioinformatics
- Molecular Biology
Background:
- Translationally controlled tumor protein (TCTP) plays crucial roles in various cellular processes.
- Understanding TCTP from parasitic organisms like Spirometra mansoni is vital for developing novel therapeutic strategies.
Purpose of the Study:
- To predict the structure and function of Spirometra mansoni TCTP using bioinformatics tools.
- To establish a theoretical foundation for future research on SmTCTP.
Main Methods:
- Open reading frame (ORF) identification and amino acid translation of Spirometra mansoni EST sequences.
- Utilizing a suite of online bioinformatics tools (e.g., Blast, InterProScan, Phyre2) for structural and functional prediction.
- Analysis of protein properties including molecular weight, evolutionary relationships, signal peptides, transmembrane domains, and secondary structure.
Main Results:
- The identified Spirometra mansoni TCTP (SmTCTP) consists of 173 amino acid residues with a theoretical molecular weight of 19,872.0 Da.
- SmTCTP exhibits the closest evolutionary relationship to TCTP from Clonorchis sinensis and Schistosoma species.
- The protein lacks signal peptide and transmembrane domains, with a predicted secondary structure comprising two α-helices and eight β-strands.
Conclusions:
- SmTCTP possesses diverse biological functions.
- The predicted characteristics suggest SmTCTP as a potential vaccine candidate molecule.
- SmTCTP is identified as a potential drug target for therapeutic interventions against Spirometra mansoni infections.
