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Updated: May 10, 2026
Biological Compatibility Profile on Biomaterials for Bone Regeneration
Published on: November 16, 2018
The effect of low dose teicoplanin-loaded acrylic bone cement on biocompatibility of bone cement
Zekeriya Öztemür1, Zeynep Sümer, Tutku Tunç
1Cumhuriyet University School of Medicine, Department of Orthopaedic and Traumatology, Sivas, Turkey. oztemur@cumhuriyet.edu.tr
Abstract:
Antibiotic-loaded acrylic bone cement (polymethylmethacrylate, PMMA) is used to prevent or treat infection in total joint replacement surgery. The purpose of this study was to investigate biocompatibility and cytotoxicity of the teicoplanin-loaded acrylic bone cement. Cytotoxicity examination of acrylic bone cement balls and 400 mg teicoplanin added acrylic bone cement balls conducted by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. SEM (Scanning electron microscopy) was used to observe adhesion and spreading of cells on surface of the balls. Cytotoxicity examination conducted by MTT assay on acrylic bone cement balls and teicoplanin-added acrylic bone cement balls revealed no cytotoxicity. SEM analysis put forward that cells started to proliferate and adhere on surface of the samples in both groups as a result of 48-hour incubation and that the cell proliferation over acrylic bone cement and teicoplanin-added acrylic bone cement was similar. As a consequence, there was no cytotoxicity in acrylic bone cement and teicoplanin-added acrylic bone cement groups according to results of MTT assay. On the other hand, results of SEM showed that biocompatibility of both groups was similar. In conclusion, teicoplanin-loaded bone cement did not change biocompatibility of bone cement in studied dose.
Insights
Teicoplanin-loaded bone cement shows no cytotoxicity and maintains biocompatibility in total joint replacement surgery. Cell proliferation and adhesion were similar to standard bone cement, indicating safety at the tested dose.
Area of Science:
- Biomaterials Science
- Orthopedic Surgery
- Infectious Disease
Background:
- Antibiotic-loaded acrylic bone cement (polymethylmethacrylate, PMMA) is crucial for preventing and treating infections in total joint replacement.
- Evaluating the biocompatibility and cytotoxicity of antibiotic-loaded bone cement is essential for patient safety and treatment efficacy.
Purpose of the Study:
- To investigate the biocompatibility and cytotoxicity of teicoplanin-loaded acrylic bone cement.
- To assess the impact of teicoplanin on cell adhesion, proliferation, and overall biocompatibility.
Main Methods:
- Cytotoxicity was evaluated using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay.
- Cell adhesion and proliferation were observed using Scanning Electron Microscopy (SEM) on acrylic bone cement and teicoplanin-added acrylic bone cement samples.
- Samples were incubated for 48 hours to assess cell behavior.
Main Results:
- MTT assays revealed no significant cytotoxicity for either the standard acrylic bone cement or the teicoplanin-loaded bone cement.
- SEM analysis demonstrated similar cell proliferation and adhesion on the surfaces of both standard and teicoplanin-loaded bone cement samples after 48 hours of incubation.
- The results indicate comparable biocompatibility between the two groups.
Conclusions:
- Teicoplanin-loaded acrylic bone cement, at the studied dose, does not exhibit cytotoxicity.
- The addition of teicoplanin to acrylic bone cement does not adversely affect its biocompatibility, maintaining similar cell proliferation and adhesion characteristics.
- Teicoplanin-loaded bone cement is a safe option for preventing or treating infections in total joint replacement surgery without compromising biocompatibility.
