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Structure-function relationship in the CYP74 family: conversion of divinyl ether synthases into allene oxide

Yana Y Toporkova1, Valeria S Ermilova, Svetlana S Gorina

  • 1Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P.O. Box 30, Kazan 420111, Russia.

FEBS Letters
|July 6, 2013
PubMed

Keywords:
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid13-HPOT9-HPODAOSAllene oxide synthaseCYP74 familyCytochrome P450DESDivinyl ether synthaseHPLI-helix central domainIHCDIMACLinum usitatissimum DES (CYP74B16)LuDESMeNicotiana tabacum DES (CYP74D3)NtDESSRSSite-directed mutagenesisTMSWTallene oxide synthasedivinyl ether synthasehydroperoxide lyaseimmobilized metal affinity chromatographymethylsubstrate recognition sitetrimethylsilylwild type

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