Related Experiment Video
Updated: May 10, 2026

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy
Published on: December 12, 2025
Microsecond dynamics of an unfolded protein by a line confocal tracking of single molecule fluorescence
Hiroyuki Oikawa1, Yuta Suzuki, Masataka Saito
1Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba, Sendai, Miyagi 980-8577, Japan.
Abstract:
We present a new method for high speed tracking of fluorescence time series from single proteins. The method uses a fast sample flow and a modified confocal microscopy, line confocal microscopy, and achieves the time resolution of less than 20 μs. The obtained time series from the B domain of protein A labeled with donor and acceptor fluorophores suggest conformational heterogeneity and dynamic fluctuations in the unfolded state.
More Related Videos
07:56Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time
Published on: May 30, 2021
10:20Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
Published on: September 5, 2019