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Related Concept Videos

Plant Tissue Culture02:57

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
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Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture

Published on: May 26, 2016

Biomedical advances from tissue culture.

Tetsuji Okamoto1, J Denry Sato, David W Barnes

  • 1The Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, 734-8553, Japan, tetsuok@hiroshima-u.ac.jp.

Cytotechnology
|July 6, 2013
PubMed
Summary
This summary is machine-generated.

Tissue culture techniques were refined to overcome fibroblast overgrowth, enabling the isolation of functionally differentiated cell strains. This advancement supports the use of cell culture in understanding cellular behavior and developing new therapies.

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BioMEMS and Cellular Biology: Perspectives and Applications

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BioMEMS and Cellular Biology: Perspectives and Applications
16:30

BioMEMS and Cellular Biology: Perspectives and Applications

Published on: October 1, 2007

Area of Science:

  • Cell Biology
  • Biotechnology
  • Cancer Research

Background:

  • Early tissue culture studies were confounded by fibroblast overgrowth, misinterpreting cellular dedifferentiation.
  • Selective culture methods were developed to isolate functionally differentiated tumor cells.

Purpose of the Study:

  • To develop enrichment culture techniques for isolating functionally differentiated tumor cell strains.
  • To investigate the utility of cell culture in modeling in vivo cellular behavior and hormonal dependencies.

Main Methods:

  • Employing alternate animal and culture passage for selective enrichment of differentiated tumor cells.
  • Developing and utilizing hormonally defined culture media with clonal cell lines.

Main Results:

  • Successfully derived numerous functionally differentiated clonal cell strains across various cell types.
  • Demonstrated that cell culture can reveal hormonal responses and dependencies, mirroring in vivo conditions.
  • Identified hormonal dependencies in cancer cells, informing targeted therapy development.
  • Revealed hormonal responses in pluripotent cells, aiding stem cell application research.

Conclusions:

  • Tissue culture, particularly with defined media and clonal lines, accurately represents in vivo cellular states.
  • Cell culture technology is crucial for discovering cellular mechanisms and developing novel therapies for human health issues.
  • Advancements in tissue culture continue to drive progress in cancer treatment and stem cell applications.