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In-depth study of homogeneity in DBS using two different techniques: results from the EBF DBS-microsampling
Zoe Cobb1, Ronald de Vries, Neil Spooner
1Quotient Bio Analytical Sciences (part of the LGC group), Newmarket Road, Fordham, Cambridgeshire, CB7 5WW, UK. zoe.cobb@quotientbioresearch.com
Bioanalysis
|July 9, 2013
Summary
Dried blood spots (DBS) microsampling can be affected by sample inhomogeneity. Factors like substrate, compound, and hematocrit levels impact DBS homogeneity, posing a challenge for regulated bioanalysis.
Area of Science:
- Bioanalysis
- Pharmacokinetics
- Microsampling Technologies
Background:
- The European Bioanalysis Forum dried blood spots (DBS) microsampling consortium initially lacked a dedicated homogeneity team.
- Two separate experimental teams generated data relevant to DBS sample homogeneity.
Purpose of the Study:
- To investigate sample homogeneity in dried blood spots (DBS) for bioanalytical applications.
- To identify factors contributing to inhomogeneity in DBS samples.
Main Methods:
- Utilized two distinct experimental approaches: a radiolabeled and a non-radiolabeled method.
- Collected and analyzed data on DBS sample homogeneity.
Main Results:
- Complementary data from both approaches revealed significant effects on sample inhomogeneity.
- Identified substrate type, compound properties, and hematocrit levels as key factors influencing DBS homogeneity.
Conclusions:
- Sample inhomogeneity presents a substantial obstacle for the use of DBS in regulated bioanalysis.
- Further investigation into DBS inhomogeneity is crucial during method establishment, especially when the entire spot is not analyzed.
