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Update of the EBF recommendation for the use of DBS in regulated bioanalysis integrating the conclusions from the EBF

Philip Timmerman1, Steve White, Zoe Cobb

  • 1Janssen R&D NV, Turnhoutseweg, 30 B-2340 Beerse, Belgium. ptimmerm@its.jnj.com

Bioanalysis
|July 9, 2013
PubMed

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Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...