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The assignment of the genes coding for human complement components C6 and C7 to chromosome 5
S J Jeremiah1, C M Abbott, Z Murad
1MRC Human Biochemical Genetics Unit, Galton Laboratory, University College London.
Annals of Human Genetics
|May 1, 1990
Summary
Researchers identified the human genes for complement components C6 and C7. These genes are located on chromosome 5, a key finding for understanding the complement system and related genetic disorders.
Area of Science:
- Genetics
- Immunology
- Molecular Biology
Background:
- The complement system is crucial for innate immunity.
- Complement components C6 and C7 are essential for the formation of the membrane attack complex.
- The precise chromosomal location of human C6 and C7 genes was previously unconfirmed.
Purpose of the Study:
- To determine the chromosomal location of human complement components C6 and C7.
- To establish a genetic marker for C6 and C7 within the human genome.
Main Methods:
- Utilized somatic cell hybrids to map human genes.
- Employed restriction enzyme digestion and Southern blot analysis with human cDNA probes for C6 and C7.
- Applied polymerase chain reaction (PCR) to detect human C7 gene sequences.
Main Results:
- Human C6 and C7 gene sequences were detected in somatic cell hybrids.
- The presence of human C6 and C7 was consistently observed in hybrids containing chromosome 5.
- Absence of chromosome 5 correlated with the absence of C6 and C7 sequences.
Conclusions:
- Human complement components C6 and C7 are located on chromosome 5.
- Chromosome 5 serves as a reliable marker for the presence of C6 and C7 genes.
- This localization provides a foundation for further genetic studies of the complement system.