Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Inhibitors of Bacterial DNA Synthesis

Inhibitors of Bacterial DNA Synthesis

Bacterial pathogens depend on precise and efficient DNA replication to sustain infection. Two type II topoisomerases—DNA gyrase and topoisomerase IV—are critical to this process, as they resolve DNA supercoiling and unlink chromosomes during replication. Fluoroquinolones, synthetic derivatives of quinolones, exploit this mechanism by stabilizing the transient DNA–enzyme cleavage complex, preventing strand religation, and causing lethal double-strand breaks. These antibiotics are selectively...

Urinary Tract Calculi VI: Surgical Management

Urinary Tract Calculi VI: Surgical Management

Procedures for Kidney StonesMedical intervention is necessary when kidney stones or renal calculi are too large to pass spontaneously (typically greater than 5 millimeters) when stones are accompanied by symptomatic infection (such as fever or pyelonephritis), when they impair kidney function, or when they cause persistent symptoms like severe pain, nausea, or urinary retention. Additionally, patients with only one kidney or those who cannot be treated with medical management also require...

Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care

Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care

A healthcare provider can diagnose a urinary tract infection (UTI) through several methods:Medical History and Symptoms: The provider will take a detailed medical history and ask about symptoms such as frequent urination, burning sensation during urination, and lower abdominal pain.Urinalysis: A clean-catch urine sample is collected in a sterile container and tested for the presence of bacteria, white blood cells (leukocytes), nitrites, blood, and protein. The presence of leukocytes and...

Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy

Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy

Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...

Pharmaceutical Alternatives: Stability-Related Therapeutic Nonequivalence

Pharmaceutical Alternatives: Stability-Related Therapeutic Nonequivalence

Generic intravenous (IV) drugs are considered bioequivalent to their branded counterparts due to their 100% bioavailability upon administration. However, variations in stability among different drug products can significantly influence their therapeutic performance, even if they are pharmaceutically equivalent.Cefuroxime, a prophylactic antimicrobial, is often used as a single-dose IV injection for patients undergoing coronary artery bypass grafting surgery. A 3 g dose typically provides...

Urinary Tract Calculi III: Medical Management

Urinary Tract Calculi III: Medical Management

The diagnosis of renal calculi involves several imaging techniques, including non-contrast CT scans and ultrasound. These methods help visualize kidney stones, assess their size and location, and detect possible obstructions. Additionally, Measuring urine pH is useful for diagnosing specific stone types, such as struvite (alkaline pH) and uric acid stones (acidic pH). Cystine stones are primarily linked to cystinuria, a genetic condition. A urinalysis helps detect blood in the urine (hematuria)...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Invited Commentary: Robotic Cholecystectomy: Who Benefits?

Journal of the American College of Surgeons·2026

Same author

Paraesophageal Hernia Repair Outcomes in Elective, Urgent, and Emergent Patient Populations.

Journal of the American College of Surgeons·2026

Same author

Impact of 2023 Ventral Hernia Repair CPT Code Changes on Work Relative Value Units in a Tertiary Hernia Referral Center.

Journal of the American College of Surgeons·2024

Same author

Updated Markov Model to Determine Optimal Management Strategy for Patients with Paraesophageal Hernia and Symptoms, Cameron Ulcer, or Comorbid Conditions.

Journal of the American College of Surgeons·2024

Same author

The Medicare Physician Fee Schedule and surgical specialties: Reform needed to preserve access.

Surgery·2024

Same author

Elective Laparoscopic Paraesophageal Hernia Repair Leads to an Increase in Life Expectancy Over Watchful Waiting in Asymptomatic Patients: An Updated Markov Analysis.

Annals of surgery·2023

Search research articles

Related Experiment Video

Updated: May 10, 2026

Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds

Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds

Published on: August 21, 2021

Doxycycline impacts hernia repair outcomes.

Job C Tharappel¹, Sandeep K Ramineni, Drew Reynolds

¹Division of General Surgery, Department of Surgery, College of Medicine, University of Kentucky, Lexington, Kentucky 40536, USA.

The Journal of Surgical Research

|July 9, 2013

Summary

Doxycycline treatment improved incisional hernia repair strength in rats by reducing matrix metalloproteinases (MMPs) and increasing collagen 1 deposition. This suggests a potential therapeutic benefit for hernia repair.

Keywords:

Collagen Doxycycline Hernia MMP Matrix metalloproteinase

More Related Videos

Application of a New Mesh Fixation Method in Laparoscopic Incisional Hernia Repair

Application of a New Mesh Fixation Method in Laparoscopic Incisional Hernia Repair

Published on: December 23, 2022

Related Experiment Videos

Last Updated: May 10, 2026

Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds

Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds

Published on: August 21, 2021

Application of a New Mesh Fixation Method in Laparoscopic Incisional Hernia Repair

Application of a New Mesh Fixation Method in Laparoscopic Incisional Hernia Repair

Published on: December 23, 2022

Area of Science:

Biomedical Engineering
Surgical Research
Pharmacology

Background:

Incisional hernias affect up to 20% of abdominal surgeries.
Elevated matrix metalloproteinases (MMPs) and altered collagen ratios are linked to hernia formation.
Doxycycline, an MMP inhibitor, shows promise in preclinical models.

Purpose of the Study:

To investigate doxycycline's effect on MMPs, collagen subtypes, and hernia repair strength.
To evaluate doxycycline's impact in an established animal model of incisional hernia repair.

Main Methods:

Rats underwent incisional hernia repair with polypropylene mesh.
Doxycycline or saline was administered daily, with analysis at 1, 2, and 4 weeks.
MMP levels, collagen ratios, and tissue mechanical properties were assessed.

Main Results:

At 4 weeks, doxycycline reduced serum and tissue MMP levels (MMP-2, MMP-9, MMP-3).
Doxycycline increased the collagen 1 to collagen 3 ratio.
Hernia repair site distraction forces were significantly increased in the doxycycline group.

Conclusions:

Doxycycline administration enhances hernia repair strength.
This improvement is associated with reduced MMPs and increased collagen 1 deposition.
Further long-term studies are needed to confirm these findings.