Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of the Platelet Plug01:22

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
Structure and Function of Platelets01:18

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...
Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors01:20

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perinatal Semaglutide Treatment Improves Maternal Health and Mitigates Offspring Metabolic Dysfunction in a Mouse Model of Maternal Obesity.

bioRxiv : the preprint server for biology·2026
Same author

Risk stratification tool for older emergency department patients: the 6-items brief geriatric assessment.

BMC geriatrics·2026
Same author

Fellowship Training After Four-Year Emergency Medicine Residency.

The western journal of emergency medicine·2026
Same author

<i>Operando</i> Nanoscale Measurements of Electrochemical Interfaces: Emerging Frontiers of Scanning Electrochemical Cell Microscopy.

Analytical chemistry·2026
Same author

Psychological and behavioral mechanisms that drive medical staff's engagement in kidney palliative care-an empirical analysis from medical staff in mainland China.

BMC health services research·2026
Same author

High Burden of Constipation Among Autistic Youth: A Nationwide Query Powered by PCORnet.

The American journal of gastroenterology·2026

Related Experiment Video

Updated: May 10, 2026

LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
06:04

LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism

Published on: April 4, 2016

Increased platelet storage time is associated with mitochondrial dysfunction and impaired platelet function.

José Paul Perales Villarroel1, Ronald Figueredo, Yuxia Guan

  • 1Division of Traumatology, Department of Surgery, Critical Care and Acute Care Surgery, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.

The Journal of Surgical Research
|July 9, 2013
PubMed
Summary
This summary is machine-generated.

Stored platelets show reduced mitochondrial function and activation after 3 days. This decline impacts their effectiveness for transfusions in trauma patients, highlighting the need for improved storage or faster usage.

Keywords:
High-resolution respirometryMitochondrial respiratory capacityPlatelet activationResuscitationSystemic organ

More Related Videos

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
04:37

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
07:06

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Published on: January 10, 2025

Related Experiment Videos

Last Updated: May 10, 2026

LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
06:04

LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism

Published on: April 4, 2016

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
04:37

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
07:06

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Published on: January 10, 2025

Area of Science:

  • Hematology
  • Cellular Biology
  • Biochemistry

Background:

  • Hemorrhagic shock from trauma often necessitates platelet transfusions for hemostasis.
  • Platelet quality may decline with prolonged storage under standard conditions.
  • Investigating the impact of storage on platelet mitochondrial function and activation is crucial.

Purpose of the Study:

  • To determine the effect of standard storage conditions on platelet mitochondrial function.
  • To assess changes in platelet activation capacity during storage.
  • To investigate the relationship between mitochondrial function and platelet activation over time.

Main Methods:

  • Platelets from 5 healthy donors were stored for up to 7 days.
  • High-resolution respirometry measured mitochondrial respiration.
  • Flow cytometry analyzed mitochondrial reactive oxygen species, P-selectin expression, and apoptosis.

Main Results:

  • Mitochondrial respiration significantly decreased after 2 days of storage.
  • Platelet activation response to stimulation diminished by day 3.
  • Increased mitochondrial reactive oxygen species and apoptosis were observed with longer storage.

Conclusions:

  • Platelet mitochondrial function and activation response significantly decline after 3 days of storage.
  • These findings suggest potential implications for the efficacy of stored platelets in clinical transfusions.
  • Further in vivo studies are warranted to validate these results.