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Related Experiment Videos

Polyclonal B cell activation in ankylosing spondylitis.

P Barbieri1, I Olivieri, G Benedettini

  • 1Rheumatic Disease Unit, University of Pisa, Italy.

Annals of the Rheumatic Diseases
|June 1, 1990
PubMed
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Patients with ankylosing spondylitis (AS) show heightened lymphocyte responses to Staphylococcus aureus Cowan I (SAC). This hyperactivity, linked to increased immunoglobulin production, is more pronounced in active disease stages.

Area of Science:

  • Immunology
  • Rheumatology
  • Cellular Biology

Background:

  • Ankylosing spondylitis (AS) is a chronic inflammatory disease primarily affecting the axial skeleton.
  • The role of B cells and their activation pathways in AS pathogenesis remains incompletely understood.

Purpose of the Study:

  • To investigate the peripheral blood lymphocyte response to polyclonal B cell activators in patients with ankylosing spondylitis (AS).
  • To determine if specific B cell activation pathways are altered in AS and correlate with disease activity or specific patient characteristics.

Main Methods:

  • Peripheral blood lymphocytes from AS patients and healthy controls were stimulated with polyclonal B cell activators, including pokeweed mitogen, protein A, and Staphylococcus aureus strain Cowan I (SAC).
  • Lymphocyte reactivity, B cell percentages, T cell responses, T cell subpopulations, and in vitro immunoglobulin production were assessed.

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  • Patients were analyzed based on disease activity and HLA-B27 antigen status.

    • Main Results:

    • Patients with AS exhibited a significantly higher lymphocyte response to Staphylococcus aureus strain Cowan I (SAC) compared to controls.
    • This heightened SAC responsiveness was not influenced by HLA-B27 status, environmental factors, or associated diseases.
    • The response to SAC was significantly higher in patients with active AS than in those with inactive disease.
    • Normal percentages of circulating B cells and similar responses to T cell mitogens were observed in AS patients and controls.
    • Increased in vitro immunoglobulin production was associated with the heightened peripheral blood lymphocyte hyperactivity to SAC in AS patients.

    Conclusions:

    • Patients with ankylosing spondylitis display an exaggerated peripheral blood lymphocyte response specifically to Staphylococcus aureus strain Cowan I (SAC).
    • This altered B cell activation pathway is associated with increased immunoglobulin production and correlates with disease activity in AS.
    • These findings suggest a potential role for specific B cell hyperactivity in the immunopathogenesis of ankylosing spondylitis.