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Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.

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Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

Tandem-repeat proteins: regularity plus modularity equals design-ability.

Yalda Javadi1, Laura S Itzhaki

  • 1University of Cambridge Department of Chemistry, Lensfield Road, Cambridge CB2 1EW, UK.

Current Opinion in Structural Biology
|July 9, 2013
PubMed
Summary
This summary is machine-generated.

Designing proteins is challenging due to complexity. Repeat proteins offer a modular approach for engineering new molecules in medicine and biotechnology.

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Last Updated: May 10, 2026

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

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Published on: August 15, 2018

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

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Published on: May 8, 2015

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Area of Science:

  • Protein engineering
  • Biophysics
  • Biotechnology

Background:

  • Globular proteins present design challenges due to topological complexity and cooperativity.
  • Tandem repeat proteins exhibit regular, modular structures, simplifying redesign.
  • Understanding natural repeat protein biophysics is key to engineering.

Purpose of the Study:

  • To review the biophysics of natural repeat proteins.
  • To discuss progress in engineering tandem repeat proteins.
  • To explore the potential of modular protein construction for new applications.

Main Methods:

  • Review of existing literature on repeat protein biophysics.
  • Analysis of recent advancements in protein engineering.
  • Discussion of modular protein design principles.

Main Results:

  • Tandem repeat proteins are amenable to engineering due to their modular nature.
  • Knowledge of natural repeat protein biophysics informs engineering strategies.
  • Successful engineering of thermodynamics, folding pathways, and molecular recognition is demonstrated.

Conclusions:

  • Modular protein design offers a powerful strategy for creating novel biomolecules.
  • Tandem repeat proteins are versatile platforms for biotechnological and medical applications.
  • Further development in modular construction holds significant promise for future innovations.