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Updated: May 9, 2026
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Assays for the Identification of Novel Antivirals against Bluetongue Virus
Published on: October 11, 2013
Deep sequencing as a method of typing bluetongue virus isolates
Pavuluri Panduranga Rao1, Yella Narasimha Reddy, Kapila Ganesh
1Ella Foundation, Genome Valley, Turkapally, Shameerpet Mandal, Hyderabad 500078, India.
Journal of Virological Methods
|July 9, 2013
Summary
Bluetongue virus (BTV) near-complete genome sequencing was achieved using Illumina next-generation sequencing. This rapid method successfully identified the genotype and serotype of two BTV isolates, aiding disease control efforts.
Area of Science:
- Veterinary Virology
- Genomics
- Molecular Diagnostics
Background:
- Bluetongue (BT) is a significant livestock disease in tropical and subtropical regions.
- The spread of Bluetongue virus (BTV) to temperate areas like North America and Northern Europe is a growing concern.
- Accurate and rapid BTV serotyping is crucial for disease control and tracing viral origins.
Purpose of the Study:
- To describe the near-complete genome sequencing and typing of two BTV isolates.
- To evaluate the efficacy of Illumina next-generation sequencing for BTV characterization.
- To demonstrate a rapid method for identifying BTV genotype and serotype.
Main Methods:
- Near-complete genome sequencing of two BTV isolates using the Illumina next-generation sequencing platform.
- Viral RNA isolation, fragmentation, reverse transcription, and cDNA library preparation with multiplex oligo ligation.
- Genome library amplification and sequencing (single and paired-end), followed by de novo and reference-based assembly.
Main Results:
- Obtained near-complete sequences for all BTV genome segments with over 20× coverage.
- Demonstrated that single-read sequencing was sufficient for accurate BTV genotype and serotype identification.
- Successfully typed the two study viruses as BTV-1 and BTV-9E.
Conclusions:
- Illumina next-generation sequencing provides a rapid and effective method for near-complete BTV genome sequencing.
- This approach facilitates efficient BTV typing, essential for disease surveillance and management.
- The study confirms the utility of advanced sequencing technologies in characterizing economically important animal viruses.
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