Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrosion Damage in Retrieved CoCrMo/Ti-6Al-4V Modular Junctions in Femoral Revision Total Knee Components.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
Same author

Metal Fatigue Fracture After Revision Total Knee Arthroplasty: A Retrieval Analysis.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
Same author

In Service to the Journal of Biomedical Materials Research-Part B: Applied Biomaterials.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
Same author

Optimization of lesser tuberosity osteotomy repair: a biomechanical assessment of suture tensioning, repair configuration, and type of suture.

JSES international·2026
Same author

In Vivo Corrosion of Acetabular Modular Tapers Reduces Local Corrosion Resistance: Near-Field Electrochemical Impedance Spectroscopy as an Indicator of the Type and Severity of Corrosion in Retrieved Hip Implants.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
Same author

Electrocautery Alters the Near-Surface Microstructure, Phases, Composition, and Hardness of Ti-6Al-4V.

Journal of biomedical materials research. Part A·2026

Related Experiment Video

Updated: May 9, 2026

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy
10:01

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Published on: May 1, 2017

Study of cellular dynamics on polarized CoCrMo alloy using time-lapse live-cell imaging.

Morteza Haeri1, Jeremy L Gilbert

  • 1Department of Biomedical and Chemical Engineering, Syracuse University, 303C Bowne Hall, Syracuse, NY 13244, USA; Syracuse Biomaterials Institute, Syracuse University, Syracuse, NY 13244, USA.

Acta Biomaterialia
|July 9, 2013
PubMed
Summary

Metallic implant surface voltage impacts cell behavior. Cathodic polarization (-400 mV) caused cell shrinkage and adhesion loss, while anodic polarization (+500 mV) led to rapid cell death due to oxidation.

Keywords:
Cathodic polarizationCell dynamicsCoCrMoTime-lapse

More Related Videos

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
10:26

Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix

Published on: December 22, 2011

Related Experiment Videos

Last Updated: May 9, 2026

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy
10:01

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Published on: May 1, 2017

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
10:26

Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix

Published on: December 22, 2011

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Surface Chemistry

Background:

  • Biocompatibility of metallic implants depends on surface interactions with the body.
  • Surface potential and redox reactions at implant interfaces influence cell adhesion and survival.
  • A specific voltage viability range exists for metallic implants to ensure cellular health.

Purpose of the Study:

  • To investigate cellular dynamics and focal adhesion changes on polarized CoCrMo alloy surfaces.
  • To determine the effects of cathodic and anodic polarization on pre-osteoblast behavior and viability.
  • To assess nucleus dynamics and reactive oxygen species (ROS) release under different polarization conditions.

Main Methods:

  • MC3T3-E1 pre-osteoblasts cultured on CoCrMo alloy surfaces polarized at -400 mV and +500 mV (Ag/AgCl).
  • Analysis of cellular dynamics (size, polarity, movement) and focal adhesion complexes.
  • Monitoring of nucleus dynamics and reactive oxygen species (ROS) release at -1000, -400, and +500 mV (Ag/AgCl).

Main Results:

  • At -400 mV (cathodic), cells showed gradual adhesion loss and shrinkage over 24 hours.
  • At +500 mV (anodic), cells became non-viable within 5 hours due to oxidation and metal ion release (e.g., Cr6+).
  • At -1000 mV, nucleus size decreased rapidly, leading to complete cell non-viability within 15 minutes. No significant ROS release was detected.

Conclusions:

  • Surface polarization significantly affects metallic implant biocompatibility and cell viability.
  • Cathodic polarization can lead to cell detachment, while anodic polarization promotes rapid cell death.
  • Understanding the voltage viability range is critical for designing safer and more effective metallic biomedical implants.