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ABC Transporters: Exporter

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ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...

ABC Transporters: Importer

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ATP-binding cassette or ABC transporters are a class of ATP-driven pumps that hydrolyze ATP to move solutes across the membrane. They can be grouped into importers and exporters. While exporters are present in all domains of life, importers exist only in bacteria and some plants.
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Protein Complexes with Interchangeable Parts

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Flippase
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Related Experiment Video

Updated: May 9, 2026

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

Published on: February 1, 2010

Mapping the functional yeast ABC transporter interactome.

Jamie Snider¹, Asad Hanif, Mid Eum Lee

¹Donnelly Centre, University of Toronto, Toronto, Ontario, Canada.

Nature Chemical Biology

|July 9, 2013

Summary

This summary is machine-generated.

This study maps the protein interactions of ATP-binding cassette (ABC) transporters in yeast, revealing diverse cellular functions and regulatory roles. The findings provide a comprehensive interactome resource for understanding ABC transporter biology.

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Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

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Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

ATP-binding cassette (ABC) transporters are crucial integral membrane proteins with significant roles in human health and disease.
Understanding the protein interactions of ABC transporters is key to elucidating their cellular functions and pharmacological relevance.

Purpose of the Study:

To comprehensively map the protein interactome of non-mitochondrial ABC transporters in Saccharomyces cerevisiae.
To integrate new interaction data with existing databases for a holistic view of ABC transporter associations.
To investigate the role of these interactions in regulating ABC transporters and modulating zinc homeostasis.

Main Methods:

Utilized membrane yeast two-hybrid technology to identify protein-protein interactions.
Integrated newly generated data with the BioGRID database for a comprehensive interactome.

Main Results:

Identified diverse functional associations for ABC transporters.
Demonstrated physical interactions among ABC transporters, suggesting self-regulation.
Revealed ABC transporter interactions involved in zinc homeostasis pathways.

Conclusions:

The generated interactome provides a valuable resource for understanding ABC transporter cellular roles and regulation.
Physical interactions play a significant role in the functional network of ABC transporters.
This work deepens the fundamental understanding of ABC transporter biology and its implications.