Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Microbiological Quality of Ready-to-Eat Salad and Salad Components Collected from Retail and Catering Premises in England and Northern Ireland.

Journal of food protection·2026
Same author

Acceptability and Feasibility of Longitudinal Sampling for Sexually Transmitted Enteric Infections in Gay, Bisexual, and Other Men Who Have Sex With Men (GBMSM): Prospective Cohort Pilot Study Conducted in 2022 in South East England.

JMIR public health and surveillance·2026
Same author

Mind the gap! Typing <i>Escherichia coli</i> O157:H7 in the pre- and post-genomic revolution eras.

Microbial genomics·2026
Same author

A 5-year review of <i>Helicobacter pylori</i> isolated from gastric biopsies at the UK Health Security Agency Gastrointestinal Bacteria Reference Unit.

Journal of medical microbiology·2026
Same author

The Third Study of Infectious Intestinal Disease (IID3 Study) in the Community: Protocol for UK-Based Prospective Cohort Studies Investigating the Disease Burden.

JMIR research protocols·2026
Same author

Public health surveillance of <i>Vibrio cholerae</i> in travellers returning to the United Kingdom.

Journal of medical microbiology·2026
Same journal

Suggestive associations between genetically predicted gut microbiota and endometriosis: a two-sample Mendelian randomization study.

Journal of medical microbiology·2026
Same journal

Whole-genome sequencing of <i>Listeria monocytogenes</i> from maternal and neonatal clinical isolates in Kuwait.

Journal of medical microbiology·2026
Same journal

The Dynamiker cryptococcal antigen lateral flow assay is an accurate, rapid and cost effective test comparable to culture for detection of cryptococcal meningitis.

Journal of medical microbiology·2026
Same journal

R pyocin sensitivity of <i>Pseudomonas aeruginosa</i> clinical isolates from different disease types.

Journal of medical microbiology·2026
Same journal

Investigation of the efficacy of high-dose flucloxacillin therapy for borderline oxacillin-resistant (BORSA) <i>Staphylococcus aureus</i> infections of <i>Galleria mellonella</i> larvae.

Journal of medical microbiology·2026
Same journal

Corrigendum: Cases of high consequence infectious diseases identified in the UK, 1962-2023.

Journal of medical microbiology·2026
See all related articles

Related Experiment Video

Updated: May 9, 2026

Screening and Isolation of C-Glycoside-Cleaving Intestinal Bacteria
06:38

Screening and Isolation of C-Glycoside-Cleaving Intestinal Bacteria

Published on: February 28, 2025

Universal extraction method for gastrointestinal pathogens.

Fenella D Halstead1,2, Adele V Lee3,4, Xose Couto-Parada4

  • 1PHE Microbiology Services, Colindale, London, UK.

Journal of Medical Microbiology
|July 9, 2013
PubMed
Summary
This summary is machine-generated.

A new universal stool extraction method using bead beating enhances nucleic acid recovery from gastrointestinal pathogens. This cost-effective approach improves diagnostics for bacteria, parasites, and viruses.

More Related Videos

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
07:24

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Published on: December 10, 2014

Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria
09:47

Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria

Published on: August 13, 2021

Related Experiment Videos

Last Updated: May 9, 2026

Screening and Isolation of C-Glycoside-Cleaving Intestinal Bacteria
06:38

Screening and Isolation of C-Glycoside-Cleaving Intestinal Bacteria

Published on: February 28, 2025

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
07:24

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Published on: December 10, 2014

Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria
09:47

Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria

Published on: August 13, 2021

Area of Science:

  • Microbiology
  • Molecular Biology
  • Diagnostic Science

Background:

  • Nucleic acid (NA) recovery from stool is crucial for diagnosing gastrointestinal infections.
  • Existing methods are often target-specific, limiting universal application.
  • A previously developed method using bead beating was effective for bacteria and parasites but not viruses.

Purpose of the Study:

  • To develop and validate a universal stool extraction method for NA recovery from diverse gastrointestinal pathogens, including viruses.
  • To assess the efficacy of bead beating as a mechanical disruption technique for broad pathogen NA extraction.
  • To evaluate the method's suitability for rapid diagnostics, as initially intended for the London 2012 Olympics.

Main Methods:

  • Mechanical disruption of stool samples using bead beating.
  • Automated nucleic acid extraction and purification.
  • Detection of target pathogens (bacteria, parasites, viruses) using real-time PCR.
  • Comparison of cycle threshold (Ct) values to assess NA recovery yields.

Main Results:

  • Bead beating significantly improved NA yields for bacterial and parasitic targets.
  • The bead-beating method proved suitable for the recovery of viral nucleic acids.
  • The universal method demonstrated robust recovery of Cryptosporidium spp. and other targets.
  • Validation involved processing sample panels containing known targets in triplicate.

Conclusions:

  • The developed universal stool extraction method, incorporating bead beating, is effective for recovering nucleic acids from bacteria, parasites, and viruses.
  • This streamlined approach offers significant cost and time savings for pathogen characterization in faecal samples.
  • The universal method enhances the potential for rapid and comprehensive gastrointestinal pathogen diagnostics.