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Related Concept Videos

Response Surface Methodology01:16

Response Surface Methodology

Response Surface Methodology (RSM) is a collection of statistical and mathematical techniques used to develop, improve, and optimize processes. It is particularly valuable when many input variables or factors potentially influence a response variable.
The process of RSM involves several key steps:
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Rab Cascades01:25

Rab Cascades

Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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Related Experiment Video

Updated: May 9, 2026

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

From gap analysis to solution and action: the RNAS⁺ model.

Xiao-Nong Zhou1, Remigio Olveda2, Banchob Sripa3

  • 1National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, People's Republic of China; Key Laboratory on Biology of Parasite and Vector, Ministry of Health, People's Republic of China, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, People's Republic of China.

Acta Tropica
|July 9, 2013
PubMed
Summary
This summary is machine-generated.

The Regional Network for Asian Schistosomiasis and other Helminth Zoonoses (RNAS(+)) enhances regional collaboration for parasitic disease control. It provides research, strategic advice, and resource mobilization for schistosomiasis and helminth infections in Southeast Asia.

Keywords:
Helminth ZoonosesNeglected tropical diseasesRNAS(+)Regional NetworkSchistosomiasisSoutheast Asia

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Area of Science:

  • Tropical medicine
  • Parasitology
  • Public health

Background:

  • The Regional Network for Asian Schistosomiasis and other Helminth Zoonoses (RNAS(+)) has operated for 12 years.
  • It evolved from an information exchange forum to a key advisory body.

Purpose of the Study:

  • To strengthen collaboration among control authorities in endemic Asian countries.
  • To foster research capacity and networking for parasitic disease control.
  • To mobilize resources for multi-country projects on helminth zoonoses.

Main Methods:

  • Developing a virtual platform for internet-based connectivity.
  • Implementing technical standardization for research.
  • Establishing accessible information databases for members.

Main Results:

  • RNAS(+) now regularly advises on regional strategies.
  • The network contributes to resource mobilization for parasitic disease projects.
  • It focuses on boosting research and alleviating disease and poverty.

Conclusions:

  • RNAS(+) plays a vital role in regional parasitic disease control efforts.
  • The network's virtual structure facilitates information sharing and collaboration.
  • Continued focus on research and capacity building is crucial for endemic areas.