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Related Experiment Video

Updated: May 9, 2026

An Adapted Optical Density-Based Microplate Assay for Characterizing Actinobacteriophage Infection

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Published on: June 30, 2023

Bacteriophage PRD1 batch experiments to study attachment, detachment and inactivation processes.

Gholamreza Sadeghi¹, Jack F Schijven, Thilo Behrends

¹Department of Environmental Health Engineering, Zanjan University of Medical Sciences, Zanjan, Iran.

Journal of Contaminant Hydrology

|July 9, 2013

Summary

Kinetic batch experiments for virus removal in subsurface environments are as laborious as column experiments. Mixing conditions significantly impact attachment rates, questioning the advantage of batch over column studies for upscaling virus transport data.

Keywords:

Bacteriophage PRD1 Batch experiment Colloids

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Following Cell-fate in E. coli After Infection by Phage Lambda

Following Cell-fate in E. coli After Infection by Phage Lambda

Published on: October 14, 2011

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Following Cell-fate in E. coli After Infection by Phage Lambda

Following Cell-fate in E. coli After Infection by Phage Lambda

Published on: October 14, 2011

Area of Science:

Environmental science
Microbiology
Water resource management

Background:

Virus contamination of water resources poses significant risks.
Subsurface environments are crucial for water purification.
Accurate assessment of virus removal is vital for protection measures.

Purpose of the Study:

To evaluate the utility of kinetic batch experiments for virus removal studies.
To investigate how batch experiment designs affect attachment, detachment, and inactivation rate coefficients.
To compare findings from batch experiments with column experiments for virus transport modeling.

Main Methods:

Conducted kinetic batch experiments using quartz sand, bacteriophage PRD1, and controlled water chemistry.
Varied container size, sand-water ratio, and mixing methods (rolling vs. tumbling).
Fitted analytical solutions of kinetic model equations to experimental data to derive rate coefficients.

Main Results:

Attachment rate coefficients were higher under tumbling (better mixing) than rolling conditions.
Increased sand-water ratio led to higher attachment rates.
Detachment rate coefficients increased linearly with the solid-water ratio under tumbling.
Collision efficiencies differed between batch and column experiments, influenced by mixing.

Conclusions:

Kinetic batch experiments are as labor-intensive as column experiments.
Upscaling virus attachment rates from batch to column requires understanding mixing dynamics.
The direct advantage of kinetic batch experiments over column experiments for virus transport studies is questionable.