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Related Experiment Video

Updated: May 9, 2026

Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology
11:20

Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology

Published on: January 24, 2018

Mimer: an automated spreadsheet-based crystallization screening system.

Ditlev Egeskov Brodersen1, Gregers Rom Andersen, Christian Brix Folsted Andersen

  • 1Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10c, DK-8000 Aarhus C, Denmark. deb@mb.au.dk

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
|July 9, 2013
PubMed
Summary
This summary is machine-generated.

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This study introduces a low-cost system for macromolecular crystallization. It simplifies the preparation of screening conditions using a spreadsheet and automated liquid handling, making structural biology more accessible.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Crystallography

Background:

  • Macromolecular crystallization is crucial for determining protein structures.
  • Commercial systems for preparing crystallization conditions can be expensive and complex.

Purpose of the Study:

  • To describe a simple, low-cost alternative for preparing macromolecular crystallization conditions.
  • To enable rapid calculation and custom design of crystallization screens.

Main Methods:

  • Utilized an intuitive spreadsheet-based approach for calculating pipetting volumes.
  • Incorporated incomplete-factorial and grid-screen designs for custom screen generation.
  • Employed a generic, inexpensive liquid handler for automated dispensing.
Keywords:
crystallization screeninggrid screenincomplete factorialprotein crystallizationsoftwarespreadsheet

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Last Updated: May 9, 2026

Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology
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Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology

Published on: January 24, 2018

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
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Published on: January 16, 2021

Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening
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Main Results:

  • The system allows for rapid and accurate calculation of reagent volumes.
  • Custom crystallization screens can be automatically designed.
  • Automated dispensing ensures reproducibility and efficiency.

Conclusions:

  • This low-cost system provides an accessible alternative to commercial setups.
  • The approach streamlines the process of preparing macromolecular crystallization screens.
  • It facilitates structural biology research by reducing cost and complexity.