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Published on: May 19, 2023
Downregulated lipid metabolism in differentiated murine adipocytes by procyanidins from defatted grape seed meal
Younghwa Kim1, Youngmin Choi, Junsoo Lee
1Department of Food Science and Technology, College of Agriculture, Chungbuk National University, Cheonju 361-763, Republic of Korea.
Abstract:
Recent reports have proposed possible anti-obesity mechanisms for antioxidants involving increased energy expenditure, pre-adipocyte differentiation and proliferation, decreased lipogenesis and increased lipolysis, and fat oxidation. The aim of this study was to examine and to confirm the anti-obesity effect of the oligomeric and polymeric procyanidin fractions from defatted grape seeds. The lipid metabolism-related mRNA level in the mouse preadipocytes, 3T3-L1 cells, was determined to evaluate the anti-obesity effect of the phenolic fractions from a grape seed meal. Lipid accumulation was reduced by 19% of the control level by the procyanidin fraction originating from the grape seed meal. Emerging from the effect of the treatment on HSL and LPL mRNA expression, lipolytic enzyme activity was not involved in the anti-obesity effects of CPE and FPP from the defatted grape seed meal. We tested and confirmed in this study the effect of the biological activities of oligomeric and polymeric procyanidins from the defatted grape seed meal. It is suggested from the results of this brief study that further studies would be desirable to focus on the anti-obesity effect of the purified extracts of a defatted grape seed meal.
Insights
Grape seed procyanidin fractions show anti-obesity effects by reducing lipid accumulation in mouse cells. These compounds may offer a natural approach to weight management, warranting further investigation into purified extracts.
Area of Science:
- Nutritional Science
- Biochemistry
Background:
- Antioxidants are investigated for anti-obesity mechanisms, including effects on energy expenditure and fat metabolism.
- Grape seed extracts contain procyanidins with potential health benefits.
Purpose of the Study:
- To confirm the anti-obesity effects of oligomeric and polymeric procyanidin fractions from defatted grape seeds.
- To evaluate the impact of these fractions on lipid metabolism in mouse preadipocytes.
Main Methods:
- Treatment of 3T3-L1 mouse preadipocytes with grape seed procyanidin fractions.
- Measurement of lipid metabolism-related mRNA levels.
- Assessment of lipid accumulation.
Main Results:
- Procyanidin fractions from defatted grape seeds significantly reduced lipid accumulation by 19% compared to controls.
- Lipolytic enzyme activity, assessed via HSL and LPL mRNA expression, was not the primary mechanism for the observed anti-obesity effects.
Conclusions:
- Oligomeric and polymeric procyanidins from defatted grape seeds exhibit anti-obesity properties.
- Further research on purified extracts is recommended to explore their full therapeutic potential for obesity management.
