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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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N-terminal labeling of peptides by trypsin-catalyzed ligation for quantitative proteomics

Yanbo Pan1, Mingliang Ye, Liang Zhao

  • 1CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Angewandte Chemie (International Ed. in English)
|July 9, 2013
PubMed
Abstract

No abstract available in PubMed .

Keywords:
enzyme catalysisisotopic labelingpeptidesquantitative proteomicsterminal labeling

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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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