Detection of anisakid larvae in cod fillets by UV fluorescent imaging based on principal component analysis and gray...

Anisakid larvae are regarded as an important hazard in marine products, and demand is growing for on-line nondestructive analytical techniques for effective monitoring of these parasites. A UV fluorescent imaging was developed for detection of the third-stage anisakid larvae in marine fishes, and different processing methods were investigated and optimized based on principal component and gray value analyses. Using cod fillets as samples, the efficiency of the developed technique was evaluated, and the overall detection ratio was greater than 80 % . These results indicate a promising application of UV fluorescent imaging as an effective and nondestructive technique for identification of anisakid larvae in fishery products.