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Red algae, also known as rhodophytes, are primarily found in marine environments, though some species inhabit freshwater and terrestrial ecosystems. These organisms exist in both unicellular and multicellular forms, with some multicellular varieties reaching macroscopic sizes.As phototrophic organisms, red algae contain chlorophyll a; however, their chloroplasts lack chlorophyll b. Instead, they possess phycobiliproteins, which serve as major light-harvesting pigments, similar to those found in...
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The group Stramenopiles include some phototrophic microorganisms. Members of this group possess flagella covered in numerous short, hairlike extensions, a feature that inspired the group's name, derived from the Latin words for "straw" and "hair." Some of the main categories of Stramenopiles include diatoms, golden algae, and brown algae.Diatoms are unicellular, photosynthetic eukaryotes, with over 200 known genera. They play a key role in the planktonic communities of both marine and...
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Freshwater microalgae harvested via flocculation induced by pH decrease.

Jiexia Liu1, Yi Zhu1, Yujun Tao1

  • 1Department of Chemistry, Jinan University, Tianhe District, Guangzhou 510632, China.

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Summary

Harvesting microalgae for biofuels is now more economical and efficient. A new pH-induced flocculation method achieves over 90% efficiency for freshwater microalgae, enabling cost-effective biofuel production.

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Area of Science:

  • Biotechnology
  • Renewable Energy

Background:

  • Microalgae are a promising source for biofuel production.
  • Efficient harvesting remains a significant challenge for large-scale microalgae cultivation.
  • Current harvesting methods face limitations in cost-effectiveness and efficiency.

Purpose of the Study:

  • To develop an economical and efficient method for harvesting freshwater microalgae.
  • To investigate the effectiveness of pH-induced flocculation for microalgae harvesting.
  • To assess the viability and growth of microalgae post-harvesting.

Main Methods:

  • Developed a novel flocculation technique by decreasing the pH of the growth medium.
  • Tested the method on high biomass concentrations of freshwater microalgae (>1g/L).
  • Assessed flocculation efficiency, mechanism, and cell viability using Evans Blue assay.

Main Results:

  • Achieved over 90% flocculation efficiency for multiple microalgae species at pH 4.0.
  • Identified charge neutralization as the primary flocculation mechanism.
  • Demonstrated minimal cell damage and maintained growth yield after pH neutralization and nutrient addition.

Conclusions:

  • The pH-induced flocculation method is economical, efficient, and simple for harvesting microalgae.
  • The method effectively treats high biomass concentrations and allows for medium recycling.
  • This approach has the potential to overcome harvesting bottlenecks and promote microalgae biofuel applications.