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Updated: Sep 16, 2026

In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
Published on: October 20, 2010
Ribonuclease activity of Entamoeba histolytica
Abstract:
Ribonuclease activity of Entamoeba histolytica was purified 65-fold by calcium phosphate gel, ammonium sulphate and acetone treatments. The enzyme was inhibited by metal ions, like Mg-2+, Mn-2+, Co-2+ and Ca-2+. Metal chelating and sulphydryl agents showed no effect. EDTA and alpha, a'-dipyridyl reversed the inhibition produced by Mg-2+, Mn-2+ and Fe-2+. Various sodium salts had negligible effect on the enzyme; however, sodium chloride activated the enzyme slightly. Amoebicidal drugs like enterovioform and chloroquine phosphate inhibited the enzyme completely, while emetine showed marked inhibitory effect. Neomycin exhibited a slight stimulatory action on this enzyme.
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