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Updated: May 9, 2026

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
Published on: November 23, 2021
Design, synthesis and cellular dynamics studies in membranes of a new coumarin-based "turn-off" fluorescent probe
Olimpo García-Beltrán1, Natalia Mena, Osvaldo Yañez
1Department of Chemistry, Faculty of Sciences, University of Chile, Santiago, Chile; Departamento de Ciencias Químicas, Facultad de ciencias Exactas, Universidad Andrés Bello, Avenida República 275, Piso 3, Santiago, Chile.
Abstract:
A new coumarin-based 'turn-off' fluorescent probe, 7-(diethylamino)-N-(1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl)-2-oxo-2H-chromene-3-carboxamide (AGD) was synthesized. This compound is highly selective for ferrous ions (Fe(2+)) and can reversibly detect them in aqueous medium. The probe localizes to the cell membrane in living cells, where it can detect changes in Fe(2+) concentration. Molecular dynamics (MD) simulations indicate that AGD interacts with the lipid bilayer at the level of the glycerol moieties.

