Quantifying proteomes and their post-translational modifications by stable isotope label-based mass spectrometry

Area of Science:

• Proteomics and Mass Spectrometry
• Systems Biology
• Translational Science

Background:

• Stable isotope labeling is a powerful technique in proteomics.
• Label incorporation can be metabolic or chemical, each with unique advantages and limitations.

Purpose of the Study:

• To review the applications of stable isotope labeling in quantitative proteomics.
• To highlight the extension of these methods to post-translational modifications and tissue analysis.

Main Methods:

• Stable isotope labeling (metabolic and chemical).
• Mass spectrometry for quantitative analysis.
• Integration of multiple data types.

Main Results:

• Quantitative proteomics enables identification of protein interactions with nucleic acids and metabolites.
• Label-based methods applied to phosphoproteins allow construction of signaling networks.
• Tissue quantification methods show potential in translational research.

Conclusions:

• Stable isotope labeling coupled with mass spectrometry is revolutionizing protein expression studies.
• The integration of diverse data types provides systems-level biological insights.
• Quantitative mass spectrometry is emerging as a key tool in translational science.