Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Biosynthesis of methanopterin.

R H White1

  • 1Department of Biochemistry and Nutrition, Virginia Polytechnic Institute and State University, Blacksburg 24061.

Biochemistry
|June 5, 1990
PubMed
Summary
This summary is machine-generated.

This study reveals the biosynthetic pathway for methanopterin in bacteria. The pterin component originates from 7,8-dihydro-6-(hydroxymethyl)pterin, with methyl groups from methionine, representing a novel coenzyme modification.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bermudagrass Dead Spot: A New Disease of Bermudagrass Caused by Ophiosphaerella agrostis.

Plant disease·2019
Same author

Predictors of post-thrombotic syndrome in a population with a first deep vein thrombosis and no primary venous insufficiency.

Journal of thrombosis and haemostasis : JTH·2013
Same author

Risk of post-thrombotic syndrome after subtherapeutic warfarin anticoagulation for a first unprovoked deep vein thrombosis: results from the REVERSE study.

Journal of thrombosis and haemostasis : JTH·2012
Same author

Comparison of the Villalta post-thrombotic syndrome score in the ipsilateral vs. contralateral leg after a first unprovoked deep vein thrombosis.

Journal of thrombosis and haemostasis : JTH·2012
Same author

The biosynthesis of n-[(2)H (7)] fatty acids byArthrobacter globiformis from [U-(2)H (15)] octanoic acid.

Lipids·2011
Same author

Residual vein obstruction as a predictor for recurrent thromboembolic events after a first unprovoked episode: data from the REVERSE cohort study.

Journal of thrombosis and haemostasis : JTH·2011

Area of Science:

  • Biochemistry
  • Microbiology
  • Molecular Biology

Background:

  • Methanopterins are essential coenzymes in methanogenic archaea.
  • Understanding their biosynthesis is crucial for comprehending microbial metabolism.

Purpose of the Study:

  • To elucidate the biosynthetic pathway of the methylated pterin in methanopterins.
  • To identify the precursors and enzymatic steps involved in methanopterin synthesis.

Main Methods:

  • Isotope labeling studies using M. volta and M. formicicum.
  • Enzyme extract assays to confirm precursor incorporation and methylation steps.

Main Results:

  • L-7,8-dihydroneopterin is cleaved to 7,8-dihydro-6-(hydroxymethyl)pterin, a direct precursor.

Related Experiment Videos

  • Methionine provides the C-11 and C-12 methyl groups of methanopterin.
  • Methanopterin biosynthesis involves coupling 7,8-H2-6-(hydroxymethyl)pterin with methaniline, followed by methylation.

    • Conclusions:

    • The pterin moiety of methanopterin is derived from 7,8-H2-6-(hydroxymethyl)pterin and methaniline.
    • This pathway represents the first known instance of coenzyme specificity modification via methylation.