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Updated: May 9, 2026
Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
Published on: June 22, 2016
[Effect of resveratrol on the activation of murine CD4(+);T lymphocytes]
Zhuolong Wang1, Xiaofeng Zeng, Mengtao Li
1Department of Rheumatology, Second Affiliated Hospital, Guangzhou Medical College, Guangzhou 510260, China. wzhuolong@163.com
Objective:
To study the effect of resveratrol on murine CD4(+);T lymphocyte activation.
Methods:
Splenic mononuclear cells (SMCs) from specific pathogen free (SPF) BALB/c mice (female, aged 9-10 weeks) were cultured with resveratrol (0, 10, 20, and 40 mmol/L) for 30 min in vitro, and then were activated with ConA, anti-CD3 or anti-CD3/anti-CD28. At 6 h (for CD69) and 36 h (for CD71), cells were harvested for being stained with anti-CD4-PerCP and anti-CD69-FITC, or anti-CD4-PerCP and anti-CD71-FITC, and the expressions of CD69 and CD71 were detected respectively using flow cytometry.
Results:
Resveratrol (≥10 mmol/L) significantly decreased CD69 levels on CD4(+);T lymphocytes activated with anti-CD3 in a dose-dependent manner (P<0.01). In addition, resveratrol (≥20 mmol/L) decreased CD69 levels on CD4(+);T lymphocytes activated with ConA, and anti-CD3/anti-CD28 in a dose-dependent manner (P<0.05). When concentrations ≥10 mmol/L, resveratrol depressed CD71 expression on ConA stimulated CD4(+);T lymphocytes (P<0.05), and resveratrol (≥20 mmol/L) decreased CD71 levels on anti-CD3 and anti-CD3/anti-CD28 stimulated CD4(+);T lymphocytes (P<0.05), all in a dose-dependent manner.
Conclusion:
Resveratrol inhibits the activation of murine CD4(+);T lymphocytes in a dosedependent manner.
