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[Effect of the insulation layer of tissue-engineering scaffold on collagen type II expression in the neo-cartilage]
Hu DA1, Yunjing Mu, Wenyong Wang
182nd Hospital of PLA, Huaian 223001, China.
Objective:
To investigate the effect of the insulation layer in the tissue-engineering composite osteochondral scaffolds on collagen type II expression in the neo-cartilage.
Methods:
Chondral phases of insulation layer-free or insulation layer-containing biphasic scaffolds were seeded with autogeneic chondrocyte-induced bone marrow-derived mesenchymal stem cells. Then, the biphasic scaffolds-cells constructs were implanted into osteochondral defects of rabbits' knees. The expression of type II collagen in the tissue-engineering cartilages was evaluated by immunohistochemistry and Western blotting at 3 and 6 months after surgery, respectively. Moreover, the mRNA expression of collagen type II was also detected by qRT-PCR.
Results:
The expression of the collagen type II at both protein and mRNA levels in tissue-engineering neo-cartilages generated by the insulation layer-containing biphasic scaffold were significantly higher than those by the insulation layer-free biphasic scaffold in vivo (P<0.05).
Conclusion:
After the insulation layer is added into the osteochondral composite scaffold, the collagen type II expression in the tissue-engineering neo-cartilage can be significantly enhanced.
