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Related Experiment Video

Updated: May 9, 2026

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

Published on: June 19, 2015

"Distribution and functional identification of complex class 1 integrons".

María Paula Quiroga¹, Sonia Marina Arduino, Andrea Karina Merkier

¹Instituto de Microbiología y Parasitología Médica, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Tecnológicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, Argentina.

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases

|July 11, 2013

Summary

Complex class 1 integrons and mobile elements like ISCR1 contribute significantly to multidrug resistance in Argentinian hospitals. These elements show high dispersion and sustained presence over time in clinical isolates.

Keywords:

Complex class 1 integrons ISCR1 Recombinase bla(CTX-M-2) gene qnrB10 gene

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Area of Science:

Microbiology
Molecular Biology
Genetics

Background:

Extended-spectrum β-lactamases and quinolone resistance are linked to complex class 1 integrons in Argentina.
The ISCR1 mobile element and its Orf513 recombinase play a role in the dissemination of these resistance genes.

Purpose of the Study:

To analyze the temporal distribution of complex class 1 integrons in Argentinian nosocomial strains.
To evaluate the functionality of the Orf513 protein within the ISCR1 mobile element.
To investigate the presence and linkage of specific resistance genes (orf513, blaCTX-M-2, dfrA3b, qnrB10, blaDHA-1) to class 1 integrons.

Main Methods:

Polymerase chain reaction (PCR) and DNA sequencing were used to detect and analyze resistance genes and integrons.
451 nosocomial strains isolated between 1989 and 2010 from Buenos Aires hospitals were studied.
Experimental analysis was performed to confirm the activity of the Orf513 protein.

Main Results:

Complex class 1 integrons showed different epidemiology in fermenting (94/171) versus non-fermenting bacilli (1/280).
ISCR1::qnrB10 was detected in clinical isolates as early as 1993.
In35::ISCR1::blaCTX-M-2 was the most prevalent integron in Enterobacteriaceae, especially Proteus mirabilis.
Orf513 protein activity was experimentally confirmed, showing binding to specific DNA sequences.

Conclusions:

Complex class 1 integrons are highly dispersed and maintained over time in Argentinian nosocomial isolates.
The ISCR1 mobile element significantly contributes to multidrug resistance phenotypes through its association with class 1 integrons.
The findings highlight the sustained role of mobile genetic elements in the evolution of antibiotic resistance.