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Pristane induced changes in rat lymphocyte membrane fluidity.

J E Bly1, L R Garrett, M A Cuchens

  • 1Department of Microbiology, University of Mississippi Medical Center, Jackson 39216-4505.

Cancer Biochemistry Biophysics
|April 1, 1990
PubMed
Summary
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Pristane (2,6,10,14-tetramethylpentadecane) alters lymphoid cell plasma membrane viscosity. These changes in membrane fluidity depend on how pristane is administered and the specific lymphoid organ studied.

Area of Science:

  • Biochemistry
  • Immunology
  • Cell Biology

Background:

  • Pristane (2,6,10,14-tetramethylpentadecane) is a known environmental alkane.
  • Its potential to alter biological membranes, particularly in lymphoid cells, requires investigation.

Purpose of the Study:

  • To investigate the membrane perturbant properties of pristane.
  • To determine the impact of pristane exposure on the viscosity of lymphoid cell plasma membranes.

Main Methods:

  • Rats were administered pristane via intraperitoneal injection or diet.
  • Plasma membrane isolates from various lymphoid organs were analyzed.
  • Fluorescence polarization using 1,6-diphenyl-1,3,5-hexatriene assessed membrane viscosity.

Main Results:

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  • Pristane was significantly incorporated into lymphoid cell plasma membranes (50-89%).
  • Lymphoid membranes from pristane-treated rats showed altered viscosity compared to controls.
  • Membrane viscosity differences varied based on administration route, exposure duration, and lymphoid organ.

Conclusions:

  • Pristane acts as a membrane perturbant, altering lymphoid cell plasma membrane viscosity.
  • The extent of membrane alteration is dependent on exposure conditions and the specific lymphoid tissue.