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Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
08:46

Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field

Published on: August 2, 2011

EvaGreen based real-time RT-PCR assay for broad-range detection of hantaviruses in the field

Ilona Mossbrugger, Eva Felder, Brigitte Gramsamer

    Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
    |July 11, 2013
    PubMed
    Abstract

    No abstract available in PubMed .

    Keywords:
    Broad reactingHantavirusesQuantitative real-time PCR (qRT-PCR)Rodent field studies

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    Real Time RT-PCR02:57

    Real Time RT-PCR

    Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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