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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
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Published on: September 13, 2022

Aptamers: versatile probes for flow cytometry.

Michael Meyer1, Thomas Scheper, Johanna-Gabriela Walter

  • 1Institut für Technische Chemie, Leibniz Universität Hannover, Callinstr. 5, 30167 Hannover, Germany.

Applied Microbiology and Biotechnology
|July 11, 2013
PubMed
Summary
This summary is machine-generated.

Aptamers, or nucleic acid probes, offer high-affinity binding for flow cytometry applications. These molecules show potential to replace antibodies for cell analysis and pathogen detection.

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Published on: August 15, 2013

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Aptamers are nucleic acid oligomers selected for high-affinity and specificity to molecular targets.
  • Systematic Evolution by Exponential Enrichment (SELEX) enables aptamer selection against diverse targets, including whole cells.
  • Flow cytometry is a crucial technique for cell analysis and detection.

Purpose of the Study:

  • To review the application of aptamers as probes in flow cytometry.
  • To highlight aptamers' utility in cell-phenotyping, cancer cell line detection, and pathogen identification.
  • To discuss the future potential of aptamers, including their combination with nanoparticles.

Main Methods:

  • Selection of aptamers from random oligonucleotide libraries against specific molecular targets.
  • Application of aptamers as probes in flow cytometry for cellular analysis.
  • Integration of aptamers with nanoparticles, such as quantum dots, to create enhanced detector molecules.

Main Results:

  • Aptamers demonstrate high affinity and specificity for various targets, including small molecules, complex targets, and whole cells.
  • Aptamers have been successfully used for cell-phenotyping, detection of cancer cell lines, virus-infected cells, and pathogens via flow cytometry.
  • Combining aptamers with nanoparticles enhances detection sensitivity and affinity.

Conclusions:

  • Aptamers are versatile tools for flow cytometry, offering specific and high-affinity detection.
  • Advancements in aptamer selection and synthesis suggest they could become strong competitors to antibodies in flow cytometry.
  • Aptamers, especially when functionalized with nanoparticles, represent a promising frontier for sensitive and specific molecular detection in biological research and diagnostics.