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Related Concept Videos

The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
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Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
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PI3K/mTOR/AKT Signaling Pathway

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Related Experiment Video

Updated: May 9, 2026

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

PSMA7 directly interacts with NOD1 and regulates its function.

Liuzhong Yang1, Zheng Tang, Huiqiang Zhang

  • 1Cancer Department of First Affiliated Hospital of Xinxiang Medical College Xinxiang, Henna, China.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|July 11, 2013
PubMed
Summary

Proteasome subunit alpha type-7 (PSMA7) interacts with nucleotide-binding oligomerization domain-containing protein 1 (NOD1) in colorectal cancer. PSMA7 negatively regulates NOD1, potentially promoting tumor growth by inhibiting NOD1-mediated apoptosis and NF-κB activation.

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Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells
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Last Updated: May 9, 2026

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells
08:11

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Published on: August 7, 2021

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Immunology

Background:

  • Proteasome subunit alpha type-7 (PSMA7) is overexpressed in colorectal cancer.
  • The precise role of PSMA7 in colorectal cancer progression requires elucidation.

Purpose of the Study:

  • To investigate the interaction partners of PSMA7.
  • To elucidate the mechanism by which PSMA7 influences colorectal cancer.

Main Methods:

  • Yeast two-hybrid screening
  • Co-immunoprecipitation (IP)
  • GST-pull down assay
  • Western blotting
  • Ubiquitin assay
  • RNA interference

Main Results:

  • PSMA7 was identified to associate with nucleotide-binding oligomerization domain-containing protein 1 (NOD1).
  • PSMA7 downregulates NOD1 expression in a proteasome-dependent manner.
  • Overexpression of PSMA7 inhibited NOD1-mediated apoptosis and NF-κB activation, while PSMA7 knockdown enhanced NOD1 activity.

Conclusions:

  • PSMA7 acts as a negative regulator of NOD1.
  • PSMA7 may promote colorectal cancer progression through its inhibitory effect on NOD1.