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Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
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PSMA7 directly interacts with NOD1 and regulates its function

Liuzhong Yang1, Zheng Tang, Huiqiang Zhang

  • 1Cancer Department of First Affiliated Hospital of Xinxiang Medical College Xinxiang, Henna, China.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|July 11, 2013
PubMed
Abstract

Insights

Proteasome subunit alpha type-7 (PSMA7) interacts with nucleotide-binding oligomerization domain-containing protein 1 (NOD1) in colorectal cancer. PSMA7 negatively regulates NOD1, potentially promoting tumor growth by inhibiting NOD1-mediated apoptosis and NF-κB activation.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Immunology

Background:

  • Proteasome subunit alpha type-7 (PSMA7) is overexpressed in colorectal cancer.
  • The precise role of PSMA7 in colorectal cancer progression requires elucidation.

Purpose of the Study:

  • To investigate the interaction partners of PSMA7.
  • To elucidate the mechanism by which PSMA7 influences colorectal cancer.

Main Methods:

  • Yeast two-hybrid screening
  • Co-immunoprecipitation (IP)
  • GST-pull down assay
  • Western blotting
  • Ubiquitin assay
  • RNA interference

Main Results:

  • PSMA7 was identified to associate with nucleotide-binding oligomerization domain-containing protein 1 (NOD1).
  • PSMA7 downregulates NOD1 expression in a proteasome-dependent manner.
  • Overexpression of PSMA7 inhibited NOD1-mediated apoptosis and NF-κB activation, while PSMA7 knockdown enhanced NOD1 activity.

Conclusions:

  • PSMA7 acts as a negative regulator of NOD1.
  • PSMA7 may promote colorectal cancer progression through its inhibitory effect on NOD1.

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