RAPD-PCR analysis for molecular characterization and genotoxic studies of a new marine fish cell line derived from
L Rocco1, I V Valentino, G Scapigliati
1Department of Environmental, Biological and Pharmaceutical Sciences and Technology, Second University of Naples, Caserta, Italy, lucia.rocco@unina2.it.
Abstract:
Continuous cell lines could provide an important tool for studying epidemiology, toxicology, cellular physiology and the host-pathogen interactions. Random amplified polymorphic deoxyribonucleic acid analysis by PCR (RAPD-PCR) was used for the molecular characterization of Dicentrarchus labrax embryonic cells (DLEC) as a possible tool to detect DNA alterations in environmental genotoxic studies. We studied the DNA pattern of the DLEC fish cell line, a fibroblast-like cell line derived from European sea bass. From a total of 15 primers only six showed good discriminatory power for the amplification process on DNA samples collected from cells by three different methods (organic extraction, salting-out method and chelating agent extraction). The results obtained show that the cell line chosen for this study could be used as a possible tool for the detection of potential genotoxicity of numerous chemical compounds.
