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Updated: May 9, 2026
Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)
Published on: December 24, 2015
Can thrombin-activated platelet releasate compensate the age-induced decrease in cell proliferation of MSC?
Melanie Vogl1, Johannes Fischer, Marcus Jäger
1Department of Orthopaedics, University Hospital, Heinrich-Heine-University, Düsseldorf, Germany.
Abstract:
Mesenchymal progenitor cells (MSCs) are promising for cell-based regeneration therapies. In elderly patients a reduced proliferation of MSCs has been described. Platelet-rich plasma (PRP) contains important factors necessary for osteogenic regeneration. The aim of this study was to find out whether the age-induced decrease in cell proliferation can be compensated by the use of supernatant of centrifuged, activated PRP (tPR). MSCs of donors of three age groups (A: young, 14-16 years, B: middle age, 36-46 years, C: older, 74-83 years) were expanded with 20% FCS alone or supplemented with thrombin-activated platelet releasate (tPR) (1%, 2.5%, and 5%) or platelet-poor plasma (PPP 5%). Cell proliferation and differentiation was measured on days 0, 3, and 7. Proliferation increased significantly in groups A and B with tPR, and non-significantly in group C. The generation times of MSCs of elderly patients were significantly increased in group C compared to groups A and B. Addition of 1% or 2.5% tPR significantly reduced population doubling times of all age groups. Adding tPR stimulates the proliferation rate of MSCs independent of donor age. For juvenile and middle-aged patients this influence was significant. Cells differentiation into osteoblasts was not influenced by addition of tPR.
Insights
Platelet-rich plasma (PRP) can enhance mesenchymal progenitor cell (MSC) proliferation, potentially counteracting age-related decline. This finding offers promise for regenerative therapies in older patients.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Regenerative Medicine
Background:
- Mesenchymal progenitor cells (MSCs) are vital for tissue regeneration.
- Elderly patients exhibit reduced MSC proliferation, limiting therapeutic potential.
- Platelet-rich plasma (PRP) contains growth factors crucial for osteogenic regeneration.
Purpose of the Study:
- To investigate if activated PRP supernatant (tPR) can overcome age-related decreases in MSC proliferation.
- To assess the impact of tPR on MSCs from different age groups (young, middle-aged, elderly).
Main Methods:
- MSCs from three age groups were cultured with FCS, supplemented with tPR (1-5%) or platelet-poor plasma (PPP).
- Cell proliferation and differentiation were assessed on days 0, 3, and 7.
- Population doubling times and generation times were calculated.
Main Results:
- tPR significantly increased MSC proliferation in young and middle-aged donors.
- While not significant, tPR showed a positive trend in elderly MSC proliferation.
- tPR significantly reduced population doubling times across all age groups.
- Osteogenic differentiation of MSCs was not affected by tPR addition.
Conclusions:
- Activated PRP supernatant (tPR) stimulates MSC proliferation, independent of donor age.
- tPR may offer a strategy to improve MSC-based regenerative therapies for elderly patients.
- tPR does not influence the osteogenic differentiation capacity of MSCs.
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