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B-cell tolerance to the B-cell receptor variable regions

Johanne T Jacobsen1, Vibeke Sundvold-Gjerstad, Frode M Skjeldal

  • 1Centre for Immune Regulation, Institute of Immunology, Oslo University Hospital, Rikshospitalet, Oslo, Norway.

European Journal of Immunology
|July 11, 2013
PubMed

An enormous number of B cells with different B-cell receptors (BCRs) are continuously produced in the bone marrow. BCRs are further diversified during the germinal center reaction. Due to extensive recirculation, B cells with mutually binding BCR are likely to meet in lymphoid organs. We have addressed possible outcomes of such an encounter in vitro. B lymphoma cells were transfected with complementary BCR, one transfectant expressing an Idiotype⁺ (Id⁺) BCR and the other an anti-Id BCR. To exclude confounding effects of secreted Ig, the transfected B lymphoma cells only expressed membrane IgD. Coincubation of paired Id⁺/anti-Id lymphoma cells results in conjugate formation, signaling, activation of Caspase 3/7, and apoptosis of at least one of the two cells in the pair. Our data provide suggestive evidence for a mechanism whereby the B-cell compartment is partly purged of B cells with complementary BCRs.

Keywords:
ApoptosisB-cell receptor (BCR)IdiotypesTolerance

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