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Related Concept Videos

Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with cytoskeletal...
What are Membranes?01:24

What are Membranes?

A cell's plasma membrane demarcates the cell's borders and determines the nature of its interaction with the environment. Cells exclude certain substances, take in others, and excrete some others in controlled quantities. The plasma membrane must be flexible to allow certain cells, such as red and white blood cells, to change their shape while passing through narrow capillaries. These are the more obvious plasma membrane functions. In addition, the plasma membrane's surface carries markers that...
What are Membranes?01:54

What are Membranes?

A key characteristic of life is the ability to separate the external environment from the internal space. To do this, cells have evolved semi-permeable membranes that regulate the passage of biological molecules. Additionally, the cell membrane defines a cell’s shape and interactions with the external environment. Eukaryotic cell membranes also serve to compartmentalize the internal space into organelles, including the endomembrane structures of the nucleus, endoplasmic reticulum and Golgi...

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Updated: May 9, 2026

Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates
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Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates

Published on: March 7, 2014

Supported membrane formation, characterization, functionalization, and patterning for application in biological

Wan-Chen Lin1, Cheng-Han Yu, Sara Triffo

  • 1Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, California.

Current Protocols in Chemical Biology
|July 11, 2013
PubMed
Summary

Supported membranes, lipid bilayers on solid substrates, are crucial for studying cell interactions and membrane properties. Protocols are provided for preparing, functionalizing, and characterizing these model systems.

Keywords:
fluorescence recovery after photobleaching (FRAP)membrane functionalizationphotolithographyquantitative fluorescence measurementsmall unilamellar vesicle (SUV)supported lipid bilayersupported membrane

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Last Updated: May 9, 2026

Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates
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Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
09:19

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Published on: September 15, 2017

Area of Science:

  • Biophysics
  • Materials Science
  • Cell Biology

Background:

  • Supported membranes, continuous lipid bilayers on solid substrates, serve as vital models for investigating protein-protein and cell-cell interactions.
  • They are instrumental in studying molecular interactions at interfaces and the complex heterogeneities found in plasma membranes.
  • Advantages include controlled membrane composition and compatibility with surface-sensitive analytical techniques.

Purpose of the Study:

  • To provide comprehensive protocols for the preparation, functionalization, and characterization of supported membranes.
  • To highlight the utility of supported membranes in addressing key questions in cell biology, leveraging advances in micro- and nanotechnology.
  • To detail essential sample preparation steps for efficient vesicle fusion, a common method for creating supported membranes.

Main Methods:

  • Vesicle fusion technique for supported membrane formation.
  • Surface-sensitive microscopic and spectroscopic techniques for characterization.
  • Protocols for membrane functionalization and sample preparation.

Main Results:

  • Established detailed procedures for creating, modifying, and analyzing supported membrane systems.
  • Demonstrated the versatility of supported membranes for diverse biological and interfacial studies.
  • Highlighted the importance of meticulous sample preparation for successful vesicle fusion.

Conclusions:

  • Supported membranes are versatile and powerful tools for biological research, particularly in understanding membrane dynamics and interactions.
  • The described protocols offer a standardized approach to preparing and utilizing supported membranes for advanced studies.
  • Further integration with micro- and nanotechnology promises expanded applications in cell biology.