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Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices
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Published on: August 14, 2015

Expression and function of NUMB in odontogenesis.

Haitao Li1, Amsaveni Ramachandran, Qi Gao

  • 1Department of Orthodontics, College of Dental Medicine, Nova Southeastern University, 3200 S. University Drive, Fort Lauderdale, FL 33328, USA.

Biomed Research International
|July 11, 2013
PubMed
Summary

NUMB protein is crucial for maintaining ameloblast progenitor cells in developing teeth. It regulates the Notch1 and sonic hedgehog (Shh) signaling pathways, essential for tooth development.

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Stem Cell Biology

Background:

  • NUMB is a key protein regulating cell fate decisions in progenitor cells.
  • Understanding NUMB's role in odontogenesis is vital for regenerative dentistry and developmental studies.

Purpose of the Study:

  • To investigate the expression patterns and functions of NUMB during tooth development (odontogenesis).
  • To elucidate the molecular mechanisms by which NUMB influences ameloblast progenitor cells.

Main Methods:

  • Isolation of NUMB full-length clones from mouse dental pulp mRNA.
  • Confocal microscopy and Western blot analysis for NUMB expression.
  • Immunohistochemistry on postnatal mouse tooth germs.
  • Overexpression studies in a preameloblast cell line (HAT-7).

Main Results:

  • Identified novel NUMB sequences and confirmed strong NUMB expression in human dental pulp stem cells (hDPSC) and preameloblasts.
  • Demonstrated differential expression of NUMB isoforms across various dental tissues.
  • Showed NUMB overexpression antagonizes activated Notch 1 and downregulates sonic hedgehog (Shh) expression in preameloblasts.

Conclusions:

  • NUMB plays a critical role in maintaining the ameloblast progenitor phenotype at the cervical loop.
  • NUMB functions by downregulating activated Notch 1 and subsequently inhibiting Shh mRNA expression.
  • This suggests NUMB is a key regulator in the signaling pathways governing tooth development.