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Related Experiment Video

Updated: May 9, 2026

Semi-Quantitative Determination of Dopaminergic Neuron Density in the Substantia Nigra of Rodent Models using Automated Image Analysis

Semi-Quantitative Determination of Dopaminergic Neuron Density in the Substantia Nigra of Rodent Models using Automated Image Analysis

Published on: February 2, 2021

Reproducibility in nerve morphometry: comparison between methods and among observers.

Antônio Paulo da Costa Bilego Neto¹, Fernando Braga Cassiano Silveira, Greice Anne Rodrigues da Silva

¹Department of Neurosciences and Behavioral Neurosciences, School of Medicine of Ribeirão Preto, University of São Paulo, 14049-900 Ribeirão Preto, SP, Brazil.

Biomed Research International

|July 11, 2013

Summary

The semiautomated nerve morphometry method is reliable and reproducible across different observers. Fully automatic methods may overestimate myelin sheath size, impacting diagnostic accuracy.

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Area of Science:

Neuroscience
Histology
Biomedical Engineering

Background:

Nerve morphometry is crucial for diagnosing neurological disorders.
Reproducibility of morphometric methods is essential for reliable clinical trial data.

Purpose of the Study:

To assess the reproducibility of a semiautomated nerve morphometry method.
To compare semiautomated and fully automated morphometry techniques.
To evaluate inter-observer variability in nerve fiber analysis.

Main Methods:

Semiautomated nerve morphometry (myelinated fiber counting and measurement) was performed by three observers using KS-400 software on rat sural nerves.
Fully automated morphometry was also conducted using the same software and images.
Inter-observer reliability and comparisons between methods were analyzed.

Main Results:

No significant differences in myelinated fiber or axon size distributions were found between the three observers using the semiautomated method.
Fully automated morphometry overestimated myelin sheath area, leading to overestimation of fiber size and underestimation of axon size.
Fiber distribution overestimation by the automatic method was approximately 0.5 μm.

Conclusions:

Semiautomated nerve morphometry is a reliable and reproducible method, suitable for multi-center clinical trials and single-experiment use by multiple investigators.
The overestimation of myelin sheath size by fully automated methods may compromise diagnostic accuracy.
Semiautomated methods offer greater precision for nerve morphometry analysis.