Related Experiment Video
Updated: May 9, 2026
06:16
High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
Published on: August 13, 2011
Directed co-evolution of an endoglucanase and a β-glucosidase in Escherichia coli by a novel high-throughput
Min Liu1, Jiali Gu, Wenping Xie
1Institute of Bioengineering and State Key Laboratory of Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, PR China.
Chemical Communications (Cambridge, England)
|July 11, 2013
Abstract:
A novel high-throughput screening method for directed co-evolution of endoglucanase and β-glucosidase was constructed based on the synergy of the two enzymes using a bicistronic operon. To our knowledge, this is the first report of directed co-evolution of two cellulosic enzymes using an insoluble screening substrate via their synergistic reactions.
