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Updated: May 9, 2026
Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: January 5, 2021
[Preliminary study on the discrimination of oral mucosa pathogens Saccharomyces with metabonomics method]
Miao Li1, Xiaomin Zhang, Qingzong Si
1Dept. of Oral Medicine, School of Stomatology, Lanzhou University, Lanzhou 730000, China.
Objective:
To evaluate the feasibility of identifying oral mucosa pathogenic bacteria by comparing the metabolic profiling of Saccharomyces and try to find a convenient and rapid way to discriminate oral micro-organisms.
Methods:
Saccharomyces albicans ATCC 10231 and Saccharomyces tropicalis ATCC 13803 and Saccharomyces glabrata ATCC 15126 were respectively inoculated in sabouraud medium. The growth quantity were measured periodically by a spectrophotometer. And the growth curves of the inoculated bacteria were completed. The culture solutions in stationary phases of the three bacteria were respectively tested with 'H-nuclear magnetic resonance(1H-NMR) spectroscopy. The data of 1H-NMR spectroscopy results were analyzed by principal components analysis (PCA).
Results:
The PCA showed respectively a obvious clustering phenomena and the points of every two groups' data stayed separately. Therefore, the NMR-based metabonomics profiles could discriminate the three kinds of mucosa pathogenic bacteria.
Conclusion:
The metabonomics can be expected to be a kind of promising useful method in quick discrimination of oral pathogenic bacteria.
