[The expression and significance of the Notch signaling pathway molecules in tongue squamous cell carcinoma]
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Published on: January 12, 2020
Tonghan Zhang1, Haichao Liu, Yujie Liang
1Dept. of Oral and Maxillofacial Surgery, Branch Hospital fo Stomatology, The Affiliated Zhongshan Hsopita of Sun Yat-sen University, Zhongshan, China.
Objective:
To explore the expression of Notch signaling receptors Notchl, Notch3 and its ligand Jaggedl, Jagged2 in tongue squamous cell carcinoma (TSCC).
Methods:
mRNA and protein expression levels of tissue samples from 74 cases of tongue cancer patients and human tongue cell line Cal-27 were detected by reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry and Western blot. Its relationship with cell proliferation and clinical pathology was analyzed.
Results:
mRNA and protein expression were detected in tongue cancer tissues, adjacent tissues and cell lines. Notchl and Notch3 protein expression in tongue cancer was higher than the adjacent tissues. Jaggedl and Jagged2 protein expression in tongue cancer and adjacent tissues had no difference. Notchl and Notch3 protein had correlation with tongue cancer clinical staging. Pathway protein expression had no correlation with pathological grade, age, gender. Notchl protein expression in lymph node metastasis-positive cases was higher than in lymph node metastasis-negative cases. The expression of Notch3 and Jagged2 had correlation. Jaggedl expression grade in metastasis-positive cases was higher than in negative cases.
Conclusion:
Notch signaling molecules have active expression in TSCC and may play important roles in tongue cancer development.
Insights
Notch signaling molecules are actively expressed in tongue squamous cell carcinoma (TSCC). Notch1 and Notch3 protein levels correlate with TSCC clinical staging and metastasis, suggesting a role in tumor development.
Area of Science:
- Oncology
- Molecular Biology
- Cell Signaling
Background:
- The Notch signaling pathway is crucial for cell-to-cell communication and plays a role in various biological processes, including cancer development.
- Aberrant Notch signaling has been implicated in several human cancers, but its specific role in tongue squamous cell carcinoma (TSCC) requires further investigation.
Purpose of the Study:
- To investigate the expression patterns of Notch signaling components, specifically Notch1, Notch3 receptors, and Jagged1, Jagged2 ligands.
- To determine the correlation between the expression of these molecules and the clinicopathological features of TSCC.
Main Methods:
- Utilized reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blot to assess mRNA and protein expression.
- Analyzed tissue samples from 74 TSCC patients and the human tongue cell line Cal-27.
- Correlated molecular expression with clinical staging, metastasis, pathological grade, age, and gender.
Main Results:
- Notch1 and Notch3 protein expression were significantly higher in TSCC tissues compared to adjacent non-cancerous tissues.
- Notch1 protein levels correlated with clinical staging and lymph node metastasis.
- Jagged1 expression was higher in metastasis-positive cases, and correlations were observed between Notch3 and Jagged2 expression.
Conclusions:
- Notch signaling molecules are actively expressed in TSCC.
- These molecules may play a significant role in the development and progression of tongue squamous cell carcinoma.
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