Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched
Kamaladasan Kalidasan1, Ying Su, Xiaoyuan Wu
1Defence Medical and Environmental Research Institute, DSO National Laboratories, 27 Medical Drive, Singapore 117510, Singapore.
Chemical Communications (Cambridge, England)
|July 12, 2013
Abstract:
An α-N-acetylgalactosamine-containing quenched activity-based probe (qABP) was designed and successfully synthesized, and it was subsequently used in directed enzyme evolution experiments aided by fluorescence-activated cell sorting (FACS) for the discovery of an α-N-acetylgalactosaminidase variant with improved catalytic activity.
