Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Urinary Tract Calculi II: Pathophysiology and Clinical Manifestations01:26

Urinary Tract Calculi II: Pathophysiology and Clinical Manifestations

Renal calculi, commonly termed kidney stones, are crystalline solid masses that form in the kidneys but can occur at any point within the urinary system, encompassing the kidneys, ureters, bladder, and urethra.The pathophysiology of renal stones involves several key factors: supersaturation of the urine with stone-forming constituents, changes in urine pH, a decrease in urine volume, and the presence of substances that promote or inhibit stone formation.Supersaturation of Urine: This is the...
Urinary Tract Calculi I: Introduction01:28

Urinary Tract Calculi I: Introduction

Renal calculi, or kidney stones, are solid deposits of minerals and salts formed inside the kidneys. In medical terminology, "calculus" refers to the stone itself, while "lithiasis" describes the process of stone formation. Depending on their location within the urinary system, these stones may be classified as either urolithiasis, when situated within the urinary tract, or nephrolithiasis, when located within the kidneys. Each term signifies the specific impact of the stone.Predisposition...
Renal Regulation of Acid-Base Balance01:29

Renal Regulation of Acid-Base Balance

Metabolic reactions in the body produce nonvolatile acids, such as sulfuric acid, which generate an acid load of approximately 1 mEq of H+ per kilogram of body weight daily. Excreting H+ in the urine is essential to balance this acid load.
In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
However, the intercalated cells in...
Nephrons01:10

Nephrons

The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma happens...
Renal Corpuscle01:20

Renal Corpuscle

The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous capillaries...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Eyebrow and eyelid position in anophthalmic sockets reconstructed with conical or spherical implants: a quantitative analysis.

Arquivos brasileiros de oftalmologia·2026
Same author

SLIT/ROBO Pathway and Prostate Cancer: Gene and Protein Expression and Their Prognostic Values.

International journal of molecular sciences·2025
Same author

Vitamin E Attenuating Effects Against the Impact of the Herbicide Atrazine on the Diaphragm Muscle of Male Wistar Rats.

Journal of toxicology·2025
Same author

Ophthalmic Parameters of Healthy White-Eyed Parakeets (<i>Psittacara leucophthalmus</i>).

Journal of avian medicine and surgery·2025
Same author

Does Size Matter? A Retrospective Study Analysing the Size of PI-RADS 4 Lesions and Its Associated Prostate Cancer Positivity with Transperineal Prostate Biopsy.

Research and reports in urology·2025
Same author

Aerobic exercise acts differentially on proteins from glucose and glycogen pathways in the SOL and PL muscles of offspring rats submitted to a low-protein maternal diet.

Biochemical and biophysical research communications·2025

Related Experiment Video

Updated: May 9, 2026

5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat
08:50

5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat

Published on: July 3, 2013

Nephrocalcinosis induced by hyperoxaluria in rats.

Natália Baraldi Cunha1, Paulo Roberto Kawano, Carlos Roberto Padovani

  • 1Postgraduate Program in Bases General Surgery, Botucatu School of Medicine, São Paulo State University UNESP, Botucatu-SP, Brazil.

Acta Cirurgica Brasileira
|July 12, 2013
PubMed
Summary

The combination of ethylene glycol and vitamin D3 in rats effectively induced nephrocalcinosis, a kidney condition characterized by calcium deposits. This model proved superior for studying hyperoxaluria-induced kidney damage.

More Related Videos

Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis
07:45

Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis

Published on: February 9, 2021

A Mouse 5/6th Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy
07:52

A Mouse 5/6th Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy

Published on: November 7, 2017

Related Experiment Videos

Last Updated: May 9, 2026

5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat
08:50

5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat

Published on: July 3, 2013

Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis
07:45

Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis

Published on: February 9, 2021

A Mouse 5/6th Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy
07:52

A Mouse 5/6th Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy

Published on: November 7, 2017

Area of Science:

  • Nephrology
  • Toxicology
  • Animal Models

Background:

  • Nephrocalcinosis is a condition involving calcium deposits in the kidneys.
  • Hyperoxaluria, characterized by high oxalate levels, is a major cause of nephrocalcinosis.
  • Developing reliable animal models is crucial for understanding and treating kidney diseases.

Purpose of the Study:

  • To evaluate two distinct agents for inducing hyperoxaluria and subsequent nephrocalcinosis in a rat model.
  • To compare the efficacy of ethylene glycol alone versus a combination of ethylene glycol and vitamin D3 in inducing kidney calcification.
  • To establish an optimal experimental model for studying nephrocalcinosis.

Main Methods:

  • Forty Sprague-Dawley rats were divided into four groups, receiving different treatments including ethylene glycol and hydroxy L-proline.
  • Animals were assessed at one and four weeks, with urine and serum samples collected for analysis.
  • Kidney tissue was analyzed using histology, histomorphometry, and Proton Induced X-Ray Emission (PIXE) to quantify calcium deposits.

Main Results:

  • Hyperoxaluria was significantly elevated in the group receiving 1.25% ethylene glycol.
  • Nephrocalcinosis was significantly more pronounced in the group treated with 0.5% ethylene glycol and vitamin D3 (p<0.01).
  • Calcium deposits in renal parenchyma were substantially higher (10x at week 1, 100x at week 4) in the ethylene glycol + vitamin D3 group compared to controls.

Conclusions:

  • The combination of 0.5% ethylene glycol and vitamin D3 provides the most effective model for inducing nephrocalcinosis in rats within 28 days.
  • This model allows for robust investigation into the mechanisms and potential treatments of hyperoxaluria-induced kidney damage.
  • The findings support the use of this specific regimen for future preclinical studies on nephrocalcinosis.