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Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
Published on: December 20, 2017
Bioportides: bioactive cell-penetrating peptides that modulate cellular dynamics.
Monika Lukanowska1, John Howl, Sarah Jones
1Research Institute in Healthcare Science, University of Wolverhampton, Wolverhampton, UK.
Biotechnology Journal
|July 12, 2013
Summary
Cell-penetrating peptides (CPPs) enhance intracellular delivery and are now termed bioportides. These peptides can have intrinsic bioactivity or deliver bioactive cargo for research, diagnostics, and therapeutics.
Area of Science:
- Biochemistry
- Molecular Biology
- Drug Delivery
Background:
- Cell-penetrating peptides (CPPs) are crucial for intracellular delivery.
- Existing CPPs like Tat, penetratin, and transportan-10 improve delivery of various molecules.
- The concept of bioportides distinguishes CPPs with intrinsic bioactivity from inert vectors.
Purpose of the Study:
- To introduce and define the term bioportide.
- To categorize bioportides into rhegnylogically organised and sychnologic types.
- To highlight the potential of bioportides in drug delivery, diagnostics, and therapeutics.
Main Methods:
- Conceptual framework development for bioportides.
- Description of monomeric peptides with discontinuous pharmacophores (rhegnylogically organised).
- Description of CPP vectors conjugated to bioactive peptides (sychnologic).
Main Results:
- Bioportides can possess intrinsic bioactivity or act as carriers for impermeable bioactive peptides.
- CPPs function as address modules for targeted delivery.
- Chimeric extensions allow for cell- and tissue-selective targeting.
Conclusions:
- Bioportides offer a versatile platform for enhancing intracellular delivery.
- The design of bioportides can be tailored for specific applications.
- Bioportides hold significant promise for advancing research tools, diagnostics, and therapeutics across various pathologies.
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